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p eif2α s52  (Novus Biologicals)


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    Novus Biologicals p eif2α s52
    P Eif2α S52, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+eif2%CE%B1+s52/CaMKII+alpha%2Fbeta+%5Bp+Thr286%2C+p+Thr287%5D+Antibody+(22B1)/pmc05749517-440-5-10
    Average 93 stars, based on 7 article reviews
    p eif2α s52 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: Thioesterase-mediated control of cellular calcium homeostasis enables hepatic ER stress
    Article Snippet: Antibodies against p-CAMKII (T286) and p-eIF2α (S52) were obtained from Novus Biologicals (NB110-96869) and Enzo Life Sciences (ADI-KAP-CP131), respectively.

    Article Title: Thioesterase-mediated control of cellular calcium homeostasis enables hepatic ER stress
    Article Snippet: Antibodies against p-CAMKII (T286) and p-eIF2α (S52) were obtained from Novus Biologicals (NB110-96869) and Enzo Life Sciences (ADI-KAP-CP131), respectively.



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    rabbit anti-p-eif2α (s52  (Thermo Fisher)
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    (A) BMMs were uninfected, uninfected and NaAs treated (250 μM for 20 mins) or MNV-infected (MOI 5) for 12 hrs. The WB was immunolabelled with anti-NS7, <t>anti-p-eIF2α</t> and anti-eIF2α antibodies (B) Immunoblot analysis of uninfected, uninfected and NaAs treated (250 μM for 20 mins) or MNV-infected (MOI 5) cell lysates harvested at 3, 6, 9 and 12 h.p.i. The WB was immunolabelled with anti-NS7, anti-p-eIF2α and anti-actin antibodies. (C and D) BMM cells were either infected with MNV (MOI 5) or left uninfected and analysed for their translation using puromycin (10 μg/mL). ( C ) Immunoblot analysis of puromycin-treated (20 mins) cell lysates harvested at 3, 6, 9, 12 and 15 h.p.i. The WB was immunolabelled with anti-puromycin, anti-NS7, anti-p-eIF2α and anti-actin antibodies. ( D ) IF analysis of puromycin treated (10 μg/ml for 30 mins) cells at 6, 9 and 12 hrs post infection. Cells were stained with anti-puromycin, anti-NS5 and DAPI for the merged image. Stars indicate uninfected cells displaying high signal for anti-puromycin. Samples were analysed via the Zeiss LSM 710 confocal microscope and analysed with the ZEN software.
    Rabbit Anti P Eif2α (S52, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+eif2%CE%B1+s52/bio_rxiv__536052-195-27-38
    Average 90 stars, based on 1 article reviews
    rabbit anti-p-eif2α (s52 - by Bioz Stars, 2026-10
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    		  Buy from Supplier
    p eif2α s52  (Novus Biologicals)
    93
    Novus Biologicals p eif2α s52
    (A) BMMs were uninfected, uninfected and NaAs treated (250 μM for 20 mins) or MNV-infected (MOI 5) for 12 hrs. The WB was immunolabelled with anti-NS7, <t>anti-p-eIF2α</t> and anti-eIF2α antibodies (B) Immunoblot analysis of uninfected, uninfected and NaAs treated (250 μM for 20 mins) or MNV-infected (MOI 5) cell lysates harvested at 3, 6, 9 and 12 h.p.i. The WB was immunolabelled with anti-NS7, anti-p-eIF2α and anti-actin antibodies. (C and D) BMM cells were either infected with MNV (MOI 5) or left uninfected and analysed for their translation using puromycin (10 μg/mL). ( C ) Immunoblot analysis of puromycin-treated (20 mins) cell lysates harvested at 3, 6, 9, 12 and 15 h.p.i. The WB was immunolabelled with anti-puromycin, anti-NS7, anti-p-eIF2α and anti-actin antibodies. ( D ) IF analysis of puromycin treated (10 μg/ml for 30 mins) cells at 6, 9 and 12 hrs post infection. Cells were stained with anti-puromycin, anti-NS5 and DAPI for the merged image. Stars indicate uninfected cells displaying high signal for anti-puromycin. Samples were analysed via the Zeiss LSM 710 confocal microscope and analysed with the ZEN software.
    P Eif2α S52, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+eif2%CE%B1+s52/CaMKII+alpha%2Fbeta+%5Bp+Thr286%2C+p+Thr287%5D+Antibody+(22B1)/pmc05749517-440-5-10
    Average 93 stars, based on 1 article reviews
    p eif2α s52 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) BMMs were uninfected, uninfected and NaAs treated (250 μM for 20 mins) or MNV-infected (MOI 5) for 12 hrs. The WB was immunolabelled with anti-NS7, anti-p-eIF2α and anti-eIF2α antibodies (B) Immunoblot analysis of uninfected, uninfected and NaAs treated (250 μM for 20 mins) or MNV-infected (MOI 5) cell lysates harvested at 3, 6, 9 and 12 h.p.i. The WB was immunolabelled with anti-NS7, anti-p-eIF2α and anti-actin antibodies. (C and D) BMM cells were either infected with MNV (MOI 5) or left uninfected and analysed for their translation using puromycin (10 μg/mL). ( C ) Immunoblot analysis of puromycin-treated (20 mins) cell lysates harvested at 3, 6, 9, 12 and 15 h.p.i. The WB was immunolabelled with anti-puromycin, anti-NS7, anti-p-eIF2α and anti-actin antibodies. ( D ) IF analysis of puromycin treated (10 μg/ml for 30 mins) cells at 6, 9 and 12 hrs post infection. Cells were stained with anti-puromycin, anti-NS5 and DAPI for the merged image. Stars indicate uninfected cells displaying high signal for anti-puromycin. Samples were analysed via the Zeiss LSM 710 confocal microscope and analysed with the ZEN software.

    Journal: bioRxiv

    Article Title: Mouse Norovirus infection arrests host cell translation uncoupled from the stress granule-PKR-eIF2α axis

    doi: 10.1101/536052

    Figure Lengend Snippet: (A) BMMs were uninfected, uninfected and NaAs treated (250 μM for 20 mins) or MNV-infected (MOI 5) for 12 hrs. The WB was immunolabelled with anti-NS7, anti-p-eIF2α and anti-eIF2α antibodies (B) Immunoblot analysis of uninfected, uninfected and NaAs treated (250 μM for 20 mins) or MNV-infected (MOI 5) cell lysates harvested at 3, 6, 9 and 12 h.p.i. The WB was immunolabelled with anti-NS7, anti-p-eIF2α and anti-actin antibodies. (C and D) BMM cells were either infected with MNV (MOI 5) or left uninfected and analysed for their translation using puromycin (10 μg/mL). ( C ) Immunoblot analysis of puromycin-treated (20 mins) cell lysates harvested at 3, 6, 9, 12 and 15 h.p.i. The WB was immunolabelled with anti-puromycin, anti-NS7, anti-p-eIF2α and anti-actin antibodies. ( D ) IF analysis of puromycin treated (10 μg/ml for 30 mins) cells at 6, 9 and 12 hrs post infection. Cells were stained with anti-puromycin, anti-NS5 and DAPI for the merged image. Stars indicate uninfected cells displaying high signal for anti-puromycin. Samples were analysed via the Zeiss LSM 710 confocal microscope and analysed with the ZEN software.

    Article Snippet: Rabbit anti-eIF2α was purchased from Invitrogen; Rabbit anti-actin from Sigma-Aldrich; Mouse anti-puromycin from Kerafast Inc; Mouse anti-G3BP1, Mouse anti-GAPDH, Rabbit anti-HIS and Rabbit anti-calnexin from Abcam and Rabbit anti-p-eIF2α (S52) and Alexa Fluor-conjugated species-specific IgG were purchased from Life Technologies.

    Techniques: Infection, Western Blot, Staining, Microscopy, Software

    (A) RAW 264.7 were either uninfected or MNV-infected (MOI 5), treated with either DMSO, C16 (1 μM), ISRIB (0.5 μM) or C16+ISRIB at 1 h.p.i for 12 hrs, before cell lysate samples were obtained. Lysates were analysed via immunoblotting and immunolabelled with anti-NS7, anti-p-eIF2α or anti-actin antibodies. (B) RAW 264.7 were either uninfected or MNV-infected (MOI 5), treated with either DMSO or C16 (1 μM) at 1 h.p.i for 12 or 15 hrs. 30 mins before cell lysate samples were obtained, cells were treated with puromycin (10 μg/ml for 30 mins) and immunolabelled with anti-NS7, anti-p-eIF2α, anti-puromycin and anti-calnexin antibodies.

    Journal: bioRxiv

    Article Title: Mouse Norovirus infection arrests host cell translation uncoupled from the stress granule-PKR-eIF2α axis

    doi: 10.1101/536052

    Figure Lengend Snippet: (A) RAW 264.7 were either uninfected or MNV-infected (MOI 5), treated with either DMSO, C16 (1 μM), ISRIB (0.5 μM) or C16+ISRIB at 1 h.p.i for 12 hrs, before cell lysate samples were obtained. Lysates were analysed via immunoblotting and immunolabelled with anti-NS7, anti-p-eIF2α or anti-actin antibodies. (B) RAW 264.7 were either uninfected or MNV-infected (MOI 5), treated with either DMSO or C16 (1 μM) at 1 h.p.i for 12 or 15 hrs. 30 mins before cell lysate samples were obtained, cells were treated with puromycin (10 μg/ml for 30 mins) and immunolabelled with anti-NS7, anti-p-eIF2α, anti-puromycin and anti-calnexin antibodies.

    Article Snippet: Rabbit anti-eIF2α was purchased from Invitrogen; Rabbit anti-actin from Sigma-Aldrich; Mouse anti-puromycin from Kerafast Inc; Mouse anti-G3BP1, Mouse anti-GAPDH, Rabbit anti-HIS and Rabbit anti-calnexin from Abcam and Rabbit anti-p-eIF2α (S52) and Alexa Fluor-conjugated species-specific IgG were purchased from Life Technologies.

    Techniques: Infection, Western Blot

    During MNV infection, viral factors such as proteins and/or RNA (blue hexagon) phosphorylate eIF2α (green oval) via PKR (orange rectangle), as well as stalling translation initiation by the MNV NS3 protein, however this translational arrest is uncoupled from the PKR–p-eIF2α axis. These stalled preinitiation complexes typically aggregate with G3BP1 (grey oval) and form SGs (red cloud), however MNV viral factors sequester G3BP1 to the MNV RC (yellow circle) to promote replication. This allows the inhibition of cap-dependent host cell translation, as well as inhibiting the formation of SGs.

    Journal: bioRxiv

    Article Title: Mouse Norovirus infection arrests host cell translation uncoupled from the stress granule-PKR-eIF2α axis

    doi: 10.1101/536052

    Figure Lengend Snippet: During MNV infection, viral factors such as proteins and/or RNA (blue hexagon) phosphorylate eIF2α (green oval) via PKR (orange rectangle), as well as stalling translation initiation by the MNV NS3 protein, however this translational arrest is uncoupled from the PKR–p-eIF2α axis. These stalled preinitiation complexes typically aggregate with G3BP1 (grey oval) and form SGs (red cloud), however MNV viral factors sequester G3BP1 to the MNV RC (yellow circle) to promote replication. This allows the inhibition of cap-dependent host cell translation, as well as inhibiting the formation of SGs.

    Article Snippet: Rabbit anti-eIF2α was purchased from Invitrogen; Rabbit anti-actin from Sigma-Aldrich; Mouse anti-puromycin from Kerafast Inc; Mouse anti-G3BP1, Mouse anti-GAPDH, Rabbit anti-HIS and Rabbit anti-calnexin from Abcam and Rabbit anti-p-eIF2α (S52) and Alexa Fluor-conjugated species-specific IgG were purchased from Life Technologies.

    Techniques: Infection, Inhibition